Pichia transformation kit
WebbThe Pichia EasyComp™ Transformation Kit is a unique kit designed for the rapid preparation and transformation of competent Pichia pastoris cells. Features Competent cells can be frozen for future use, minimizing waste Competent cells can be prepared in less than 30 minutes WebbKit Contents The Pichia Expression Kit is shipped at room temperature and contains the following components. Spheroplast Module (Box 1). Store at room temperature. Reagent …
Pichia transformation kit
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http://owl.fish.washington.edu/halfshell/bu-git-repos/LabDocs/protocols/Commercial_Protocols/Invitrogen-Easyselect_Pichia_Expression_Kit.pdf WebbPichia Strains The following Pichia pastoris strains can be used with the pGAPZ and pGAPZα vectors: Pichia Strain Genotype Purpose X-33 wild type Expression of …
Webb25 okt. 2024 · As one of the most significant steroid hormone precursors, androst-1,4-diene-3,17-dione (ADD) could be used to synthesize many valuable hormone drugs. The microbial transformation of sterols to ADD has received extensive attention in recent years. In a previous study, Mycobacterium neoaurum JC-12 was isolated and converted sterols … Webb9 aug. 2016 · This gene was successfully expressed in yeast Pichia pastoris GS115 eukaryotic expression system, and the characteristics of the recombinant protein mFcALF2 were analyzed. mFcALF2 exhibited apparent antibacterial activities against Gram-negative bacteria, including Escherichia coli, Vibrio alginolyticus, Vibrio harveyi, and Vibrio …
WebbOne tube, Five Minutes, Any ORF Electra Cloning System - So easy even a child can do it. Electra Advantages Easy – 1 tube, 5 minute reaction Universal – any ORF cloned into any Electra vector. Quickly move ORFs from MOTHER vector to multiple DAUGHTER vectors Scarless – always in frame with no nucleotide scars
WebbThe Pichia EasyComp™ Transformation Kit contains sufficient reagents (Solutions I, II, and III) for 6 preparations of competent cells. Each competent cell preparation yields enough …
WebbPichia pastoris protein expression excellence - VALIDOGEN GmbH X KEEP in TOUCH Sign up to our mailing list to keep up with latest developments and information around Pichia based protein expression. GET in TOUCH X Welcome to VALIDOGEN! Would you like us to contact you personally? REQUEST NOW guts in glory gameWebb6 apr. 2024 · As a result, transformation of the modified CtPL-DM construct led to successful expression of the recombinant protein in P. pastoris (see below). Fig. 1 Comparison of Is PETase and Ct PL-DM. a Total released PET hydrolytic products of recombinant protein of Is PETase and Ct PL-DM expressed in E. coli by using GfPET as a … guts in glory free to playWebb1 aug. 2014 · Subcloning was carried out using the TOPO cloning kit (pCR2.1 TOPO vector; Life Technologies). P. pastoris wild-type X33 and SMD1168H (protease A deficient) strains, the expression vector pGAPZαB, and Easycomp Pichia transformation kit were from Invitrogen. Gel extraction was carried out using Qiagen gel extraction kits. guts internet archiveWebb4 juli 2024 · 2.2 Solutions for Cell Preparation and Transformation 1. BEDS solution: 10-mM Bicine–NaOH, pH 8.3, 3% (v/v) ethylene glycol, 5% (v/v) dimethyl sulfoxide (DMSO ), 1M sorbitol. Add 0.81-g bicine and 91 g sorbitol to 300 mL water. Mix well to dissolve. guts in play football bettingWebbThe EasySelect™ Pichia Expression Kit features multicopy selection with Zeocin™ antibiotic selection and can do almost everything the Multi-Copy Pichia Expression Kit … guts in hindihttp://owl.fish.washington.edu/halfshell/bu-git-repos/LabDocs/protocols/Commercial_Protocols/Invitrogen-Easyselect_Pichia_Expression_Kit.pdf boxupmini-poweredgexe2420WebbThe S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly … boxupnow