Phic31 attb

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August undefined, 2024

WebMay 7, 2013 · We showed in our previous paper that phiC31 integrase works very efficiently in silkworm embryos using an extra chromosomal cassette exchange reaction system; recombination between the attP and attB sites of the plasmid occurred with a high frequency in the silkworm embryos when phiC31-integrase mRNA was supplied by … WebWe use PhiC31 integrase to catalyze recombination between one or two attB sites in a recombinant DNA with three tandem attP sites that were previously inserted into specific loci in the mouse genome (See Figure below). Mouse lines with three tandem attP sites that were knocked into the Hipp11 (H11) or Rosa26 loci (Left in Figure) are zygote donor.

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WebEnter the email address you signed up with and we'll email you a reset link. WebDuring pUAST/PhiC31 integrase-mediated integration, the unique attP site in the ZH-attP-51D fly line genome recombines with the pUAST attB site in the pUAST-PrP vector forming attR and attL... bret stern productions

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WebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. WebMar 21, 2012 · Depending on the relative orientation of the attP and attB sites, the reaction can result in excision, inversion or integration of sequences between the recognition sites, and is not reversible unless an additional protein, an excisionase, is present. WebJul 21, 2006 · The stability of the attB × attP locus, well-documented with the phiC31 integrase system in bacteria and yeast 16,17, was evidenced here by the lack of rearrangement or reversion to the wild-type ... country abbreviations for netherlands

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WebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. WebThe key to the PhiC31 integrase system is non-random integration. Isolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) … bret story carson newmanWebFor installation in small housings, control cabinets and operator panels. Simple and fast wiring by means of screw terminals. Space-saving design. For integration of mechanical … brets towing

"Webin vitro transcription of phiC31 integrase mRNA. Depositor. David Stern. Article. Stern et al G3 (Bethesda). 2024 Feb 10. pii: 6526388. Insert. phiC31 integrase. Use. In vitro transcription ... Enlarge pBac{3Xtin-eGFP,w+,attB} Plasmid #182193. Purpose. EGFP driven by tinman transcription factor binding sites. Depositor. David Stern. Article ... " - Phic31 attb

Phic31 attb

WebFeb 15, 2024 · The attB site (5'-TGCGGGTGCCAGGGCGTGCCCttGGGCTCCCCGGGCGCGTACTCC-3') is one of the two … WebBackbone manufacturer Stratagene Vector type Mammalian Expression, Bacterial Expression, Mouse Targeting ; att/PhiC31 Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 30°C Growth Strain (s) DH10B Copy number High Copy Gene/Insert Gene/Insert name Neo* …

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WebJun 17, 2010 · PhiC31 integrase induces intramolecular recombination between target sites in episomal DNA in zebrafish. a Schematic of the reporter construct Tg(eab2:attP-mCitrine-attB-mCherry) containing a chimeric eab2 promoter (open arrow), the non-coding 1st exon (grey box) and 1st intron (black line) of β-actin2 followed by an attP site (open triangles), … WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB and attP 2, 3. PhiC31 integrase does...

WebSekelsky lab Drosophila phiC31 cloning plasmids w+attB Sequences w+attB Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Full (1) Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. http://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf

WebPlasmid w+attB from Dr. Jeff Sekelsky's lab contains the insert phiC31 attB, hsp70::w. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. WebSep 16, 2008 · All subsequent modifications of that gene are introduced by phiC31-mediated integration of plasmids carrying an attB attachment site and the desired mutation. This highly efficient integration results in a tandem duplication of the target locus, which is then reduced into a single copy carrying the mutation, likely by the efficient “single ...

WebpUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494) Plasmid #160577. Purpose. 35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB) Depositor. Diego Orzaez. Article. Bernabe-Orts et al Nucleic Acids Res. 2024 Apr 6;48(6):3379.

WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... bret stephens wall street journalWebSearch $34 million in missing exemptions going back four years. Change your name and mailing address. Pay Online for Free. Use your bank account to pay your property taxes … country abbreviations franceWebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … country abbreviations for chinaWebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … bret stetka cause of deathWebThe forward and reverse primers will be designed to incorporate attB1 and attB2 sites respectively, to allow clonase-mediated integration of the PCR product into a Gateway entry vector. The primers will be designed to both have a … bret swain moragaWebBacteriophage phiC31 encodes an integrase, which acts on the phage and host attachment sites, attP and attB, to form an integrated prophage flanked by attL and attR. bret swango colliersWebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ... bret strong law firm