Web产品种类. Primary antibodies (6716) Proteins and Peptides (1655) Cell lines and Lysates (1053) ELISA and Matched Antibody Pair Kits (770) Agonists, activators, antagonists and inhibitors (533) Cellular and biochemical assays (352) Proteomics tools (13) Cell and tissue imaging tools (6) WebFlow Cyt (Intra) Use at an assay dependent concentration. WB 1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 61 kDa). IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
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Web胞内染色步骤. 1. 根据上述说明固定组织样本. 2. 加入 100 µL 洗涤剂为基础的穿透剂,在室温下避光孵育 15 min. 3. 用 2 mL PBS(含 0.1% triton 或其它穿透洗涤剂)洗涤细胞,以 300 g (2,000 rpm) 的速度离心 5 min,弃去上清液,重悬剩余物中的沉淀. 4. 按照直接和间接实验 ... WebApr 22, 2015 · 有一篇关于 流式细胞仪理论的不错的综述,介绍了流式细胞仪的操作语言。此外,Labome.com还有另一篇文章涵盖了提供流式细胞仪所用特定试剂的 主要抗体供应 … Fix cells before intracellular staining to ensure stability of soluble antigens or antigens with a short half-life (see the special recommendations below for exceptions). This retains the target protein in the original cellular location. Detecting intracellular antigens requires cell permeabilization before … See more Antigens close to the plasma membrane and soluble cytoplasmic antigens will require mild cell permeabilization without fixation. Cytoskeletal, viral and some enzyme antigens usually give optimal results when fixed … See more Detecting secreted proteins can be challenging because they may degrade rapidly. Use Brefaldin A or other compounds that … See more shutters ascot