Ctll-2 ed50
WebData Figures. (A) The biological activity of Human Recombinant IL-2, ACF was tested by its ability to promote the proliferation of CTLL-2 cells. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.99 ng/mL. (B) 1 μg of Human Recombinant IL-2 ... WebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 Recombinant Human growth factor is …
Ctll-2 ed50
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WebMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. Source. E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and ... WebJun 2, 2012 · FIGURE 61.1. A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the …
WebThe EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.99 ng/mL. (B) 1 μg of … WebThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10^6 units/mg. ... natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also ...
Web1.875 x 10^4. HT-2. 2.151 x 10^4. The specific activity (units/mg) of a cytokine is typically calculated using a bioassay with one set of conditions and parameters, and a defined cell line. Due to inherent variability, the specific activity measured with a different cell line, cultivated under different conditions in a different lab, can differ ... WebThe newly developed cell lines (termed CTLL-2_NF-kB and A20_NF-kB) were used to implement a general bioassay, which was compared to established procedures for the characterization of various types of engineered cytokine products. Moreover, the new methodologies allowed the discovery of a novel strategy for cytokine potentiation, based …
WebApr 4, 2024 · The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain γc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβ and γc.
WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 … porsche boxster awdWebED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: sequential chromatography ... IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus ... porsche boxster bazarWebMar 25, 2024 · Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. ... The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of recombinant human IL-2 is 5.0 x 106 IU/mg, which is calibrated against the human IL-2 WHO International ... sharp turntableWeb>2 x 106 units/mg Example calculation with Gibco™ Human IL-2 Recombinant Protein (Cat. No. PHC0021) Note: If the ED 50 is expressed as a range, use the midpoint of the range. … sharp tv codes for remoteWebCTLL2 cells growing dependent upon the use of IL2. Actually i am agrwoing ctll2 cells and they are totally grwoing well with good cell viability. and for their culture you should do … porsche boxster cell phone holderWebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED 50 for this effect is 3 ng/mL. ... (Interleukin-2) protein, Human (60701GMP) stimulates the proliferation of CTLL-2 cells. The ED50 is 1.5 ng/mL. IL2 (Interleukin-2) protein, Human (60701GMP) was separated on SDS-PAGE under the reducing condition and visualized by Coomassie ... porsche boxster battery deadWebBackground: Interleukin 2 (IL-2) is a T cell stimulatory cytokine best known for inducing activated T cell proliferation . in vitro ... Bioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade rh IL-2. porsche boxster black edition for sale